Lazio will try to bounce back in Serie A where they welcome Genoa at the Stadio Olimpico tomorrow.
The reverse has a maturity of three consecutive victories and saw Lazio Biancoceleste sheet to fifth place in Serie A table.
Coach Delio Rossi was disappointed with his team's 1-0 loss and is looking for an immediate response from his team as they aim to continue their effort for a place in the Champions League.
"There was too much tension from us now, I told the guys a break," he said. "It 's a shame that we lost because we played very well. Now we have to be careful and not throw away good, we've done so far.
"To put Genoa in difficulty, we have to play at a frenetic pace and likelihood of attack against the odds, because they will not leave the Olimpico for the game, but playing the game
"I expect the team when you lose the opportunity of Rome, which leaves the ground is the right spirit.
"I think about the derby, when the second leg comes around.
"Tomorrow, he played for Genoa, which is one of the teams that are established as the most during the summer, are important players. They may also hope for fourth place.
"It would be a fight against one of our direct competitors, and doing well we will get back on track."
Rossi was suspended without Cristian Ledesma duo Stefan Radu while Fabio Firmani, Sebastiano Siviglia and Matuzalem injured.
Genoa coach Gian Piero Gasperini also the hope of an immediate response from his troops after their six-match unbeaten run was finally stopped by a flight to giants Juventus 4-1 last week.
Gryphon will continue to seek first win away from the Stadio Luigi Ferraris, and victory is only three times in the Lazio region, but will take confidence from that one of those wins were last season - their first victory after the 58-59 campaign.
They will look to star striker Diego Milito to help them repeat this result and the fans will be stimulated by the Genoa president Enrico Preziosi club 'hands off' warning to clubs hoping to sign the form before in Argentina.
"Real Madrid has asked for him, and I simply replied that he would not leave Genoa, Preziosi said. "I'm tired of saying: Milito to stay here.
"And for fans of Genoa I tell you like it, let's enjoy and that's all."
Milito is currently top scorer in Serie A with 10 goals to his name and will be looking to add to its impressive game this weekend.
Matteo Paro, Omar Milanetto and Francesco Modesto are injured, but returns to the team Giandomenico Mesto.
Genoa is the eighth in the league two points behind Lazio.